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Preliminary trials identify Chicabuffers 1M and 3H as candidates in-house electroporation buffers for further optimization. ( A ) Schematic overview of the post-electroporation workflow used to identify candidate buffers. ( B ) Chicabuffers and AmaxaV buffers were evaluated across five independent trials, with three transfection reactions per buffer. Filled circles indicate <t>Salpingoeca</t> <t>rosetta</t> survival in one of three transfections after 5 days of antibiotic selection.
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Preliminary trials identify Chicabuffers 1M and 3H as candidates in-house electroporation buffers for further optimization. ( A ) Schematic overview of the post-electroporation workflow used to identify candidate buffers. ( B ) Chicabuffers and AmaxaV buffers were evaluated across five independent trials, with three transfection reactions per buffer. Filled circles indicate <t>Salpingoeca</t> <t>rosetta</t> survival in one of three transfections after 5 days of antibiotic selection.
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Preliminary trials identify Chicabuffers 1M and 3H as candidates in-house electroporation buffers for further optimization. ( A ) Schematic overview of the post-electroporation workflow used to identify candidate buffers. ( B ) Chicabuffers and AmaxaV buffers were evaluated across five independent trials, with three transfection reactions per buffer. Filled circles indicate <t>Salpingoeca</t> <t>rosetta</t> survival in one of three transfections after 5 days of antibiotic selection.
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Preliminary trials identify Chicabuffers 1M and 3H as candidates in-house electroporation buffers for further optimization. ( A ) Schematic overview of the post-electroporation workflow used to identify candidate buffers. ( B ) Chicabuffers and AmaxaV buffers were evaluated across five independent trials, with three transfection reactions per buffer. Filled circles indicate <t>Salpingoeca</t> <t>rosetta</t> survival in one of three transfections after 5 days of antibiotic selection.
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Preliminary trials identify Chicabuffers 1M and 3H as candidates in-house electroporation buffers for further optimization. ( A ) Schematic overview of the post-electroporation workflow used to identify candidate buffers. ( B ) Chicabuffers and AmaxaV buffers were evaluated across five independent trials, with three transfection reactions per buffer. Filled circles indicate <t>Salpingoeca</t> <t>rosetta</t> survival in one of three transfections after 5 days of antibiotic selection.
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Preliminary trials identify Chicabuffers 1M and 3H as candidates in-house electroporation buffers for further optimization. ( A ) Schematic overview of the post-electroporation workflow used to identify candidate buffers. ( B ) Chicabuffers and AmaxaV buffers were evaluated across five independent trials, with three transfection reactions per buffer. Filled circles indicate <t>Salpingoeca</t> <t>rosetta</t> survival in one of three transfections after 5 days of antibiotic selection.
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bl21 rosetta 2  (Merck & Co)
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Preliminary trials identify Chicabuffers 1M and 3H as candidates in-house electroporation buffers for further optimization. ( A ) Schematic overview of the post-electroporation workflow used to identify candidate buffers. ( B ) Chicabuffers and AmaxaV buffers were evaluated across five independent trials, with three transfection reactions per buffer. Filled circles indicate <t>Salpingoeca</t> <t>rosetta</t> survival in one of three transfections after 5 days of antibiotic selection.
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e coli rosetta de3 cells  (Novogene)
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Preliminary trials identify Chicabuffers 1M and 3H as candidates in-house electroporation buffers for further optimization. ( A ) Schematic overview of the post-electroporation workflow used to identify candidate buffers. ( B ) Chicabuffers and AmaxaV buffers were evaluated across five independent trials, with three transfection reactions per buffer. Filled circles indicate <t>Salpingoeca</t> <t>rosetta</t> survival in one of three transfections after 5 days of antibiotic selection.
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Preliminary trials identify Chicabuffers 1M and 3H as candidates in-house electroporation buffers for further optimization. ( A ) Schematic overview of the post-electroporation workflow used to identify candidate buffers. ( B ) Chicabuffers and AmaxaV buffers were evaluated across five independent trials, with three transfection reactions per buffer. Filled circles indicate Salpingoeca rosetta survival in one of three transfections after 5 days of antibiotic selection.

Journal: bioRxiv

Article Title: An accessible transfection protocol for choanoflagellates

doi: 10.64898/2026.03.10.710884

Figure Lengend Snippet: Preliminary trials identify Chicabuffers 1M and 3H as candidates in-house electroporation buffers for further optimization. ( A ) Schematic overview of the post-electroporation workflow used to identify candidate buffers. ( B ) Chicabuffers and AmaxaV buffers were evaluated across five independent trials, with three transfection reactions per buffer. Filled circles indicate Salpingoeca rosetta survival in one of three transfections after 5 days of antibiotic selection.

Article Snippet: Salpingoeca rosetta was maintained in monoxenic co-culture with the bacterium Echinocola pacifica (co-culture “SrEpac”; American Type Culture Collection [ATCC], Manassas, VA; Cat No. PRA-390).

Techniques: Electroporation, Transfection, Selection

Chicabuffer 1M with pulse DS-137 yields the optimal combination of in-house buffer and pulse program for further fine-tuning of transfection efficiency. Plasmid-encoded nanoluciferase was transfected into S. rosetta using ( A ) Chicabuffer 1M and ( B ) Chicabuffer 3H across a range of pulse programs varying in efficiency and viability in triplicate. Nanoluciferase activity was measured 24 hours post-nucleofection and reported as luminescence relative to the average of three no-pulse controls.

Journal: bioRxiv

Article Title: An accessible transfection protocol for choanoflagellates

doi: 10.64898/2026.03.10.710884

Figure Lengend Snippet: Chicabuffer 1M with pulse DS-137 yields the optimal combination of in-house buffer and pulse program for further fine-tuning of transfection efficiency. Plasmid-encoded nanoluciferase was transfected into S. rosetta using ( A ) Chicabuffer 1M and ( B ) Chicabuffer 3H across a range of pulse programs varying in efficiency and viability in triplicate. Nanoluciferase activity was measured 24 hours post-nucleofection and reported as luminescence relative to the average of three no-pulse controls.

Article Snippet: Salpingoeca rosetta was maintained in monoxenic co-culture with the bacterium Echinocola pacifica (co-culture “SrEpac”; American Type Culture Collection [ATCC], Manassas, VA; Cat No. PRA-390).

Techniques: Transfection, Plasmid Preparation, Activity Assay